MouseScan™ Murine Proteome Phage-display

Entire Murine Proteome in a Single Tube Assay

MouseScan™, an application of Bacteriophage-Display Immunoprecipitation Sequencing (PhIP-Seq), is composed of the entire normal mouse proteome.


MouseScan™ PhIP-Seq Overview

Bacteriophage Immunoprecipitation Sequencing (PhIP-Seq) is a powerful method of multiplexed analysis that combines DNA high-throughput sequencing with next-generation proteomics

PhIP-Seq allows researchers to determine what antibodies were created by a mouse's unique history of immune exposures. PhIP-Seq is the highest-coverage antigen-specific assay yet developed, able to detect antibodies versus virtually any antigen via overlapping long peptides. MouseScan enables simultaneous epitope-level autoantibody profiling versus an entire GRCm38.p5 mouse proteome (50,135 proteins and isoforms) via phage-display and immunoprecipitation sequencing.

MouseScan Figure

MouseScan PhIP-Seq is composed of the entire normal mouse proteome (GRCm38.p5). The library contains all proteins expressed as overlapping 62-mer peptide tiles on the surface of bacteriophages. For an assay, an aliquot from this library is reacted with diluted mouse serum or other antibody-containing fluid. Bound antibodies are immunoprecipitated with protein A/G beads, the precipitate amplified by PCR, and the sequences quantified by a next-generation sequencing and analysis pipeline that compares patient-sample IP read counts to negative controls with no antibody input (mock IPs) in the context of overall clonal frequency of individual peptides in the parent library. Output data are then created at both the peptide level.

MouseScan Technology Overview